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Anti-RPL27/RPL27A Antibody (A88460)

Western Blot - Anti-RPL27/RPL27A Antibody (A88460) - Antibodies.com Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460) - Antibodies.com Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460) - Antibodies.com Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460) - Antibodies.com Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to RPL27/RPL27A for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-RPL27/RPL27A Antibody
Description
Rabbit polyclonal antibody to RPL27/RPL27A.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human RPL27 (NP_000979.1).
Sequence
MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
16 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

Western Blot - Anti-RPL27/RPL27A Antibody (A88460) - Antibodies.comEnlarge Image

Western Blot - Anti-RPL27/RPL27A Antibody (A88460)

Western blot analysis of extracts of various cell lines, using Anti-RPL27/RPL27A Antibody (A88460) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460)

Immunofluorescence analysis of C6 cells using Anti-RPL27/RPL27A Antibody (A88460) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460)

Immunofluorescence analysis of L929 cells using Anti-RPL27/RPL27A Antibody (A88460) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-RPL27/RPL27A Antibody (A88460)

Immunofluorescence analysis of U-2 OS cells using Anti-RPL27/RPL27A Antibody (A88460) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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