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Anti-RSK1 + RSK2 + RSK3 + RSK4 (phospho Ser221 + Ser227 + Ser218 + Ser232) Antibody (A94973)

Western Blot - Anti-RSK1 + RSK2 + RSK3 + RSK4 (phospho Ser221 + Ser227 + Ser218 + Ser232) Antibody (A8182) - Antibodies.com
This product is discontinued

Anti-RSK1 + RSK2 + RSK3 + RSK4 (phospho Ser221 + Ser227 + Ser218 + Ser232) Antibody (A94973) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-RSK1 + RSK2 + RSK3 + RSK4 (phospho Ser221 + Ser227 + Ser218 + Ser232) Antibody
Description
Rabbit polyclonal antibody to RSK1 + RSK2 + RSK3 + RSK4 (phospho Ser221 + Ser227 + Ser218 + Ser232).
Specificity
This antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/S218/232.
Applications
WB, ELISA
Dilutions
WB: 1:500~1:1000, ELISA: 1:40000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/S218/232 (amino acids 191-240).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
82kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-RSK1 + RSK2 + RSK3 + RSK4 (phospho Ser221 + Ser227 + Ser218 + Ser232) Antibody (A8182) - Antibodies.comEnlarge Image

Western Blot - Anti-RSK1 + RSK2 + RSK3 + RSK4 (phospho Ser221 + Ser227 + Ser218 + Ser232) Antibody (A94973)

Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30' using Anti-RSK1 + RSK2 + RSK3 + RSK4 (phospho Ser221 + Ser227 + Ser218 + Ser232) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.