RSPO3 expression in U2OS (A), A549 (B) HeLa (C), and KNRK (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-RSPO3 Antibody (A326286) at 0.3µg/ml (A-B) or 0.1µg/ml (C-D) and detected by chemiluminescence.
RSPO3 expression in Mouse Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-RSPO3 Antibody (A326286) at 0.1µg/ml and detected by chemiluminescence.
RSPO3 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-RSPO3 Antibody (A326286) at 4µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for RSPO3 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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