Western blot analysis of extracts of various cell lines, using Anti-S100P Antibody (A88205) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-S100P Antibody (A88205) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-S100P Antibody (A88205) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-S100P Antibody (A88205) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of human placenta cells using Anti-S100P Antibody (A88205) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 300µg extracts of HT-29 cells using 3µg of Anti-S100P Antibody (A88205). This Western blot was performed on the immunoprecipitate using Anti-S100P Antibody (A88205) at a dilution of 1:1000.
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