Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DCIP, Dendritic cell-derived IFNG-induced protein, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, dNTPase, hSAMHD1, Monocyte protein 5, MOP-5, MOP5, SAM domain and HD domain-containing protein 1
Western blot analysis of extracts of various cell lines, using Anti-SAMHD1 Antibody (A15901) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using Anti-SAMHD1 Antibody (A15901) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-SAMHD1 Antibody (A15901) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-SAMHD1 Antibody (A15901). This Western blot was performed on the immunoprecipitate using Anti-SAMHD1 Antibody (A15901) at a dilution of 1:1000.
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