Anti-SAPK (phospho Tyr185) Antibody (A94409)

Name: Anti-SAPK (phospho Tyr185) Antibody
Brand: Antibodies.com
SKU: A94409
Price: 115.0 GBP
Availability: InStock

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$190

Rabbit polyclonal antibody to SAPK (phospho Tyr185) for WB, IHC and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-SAPK (phospho Tyr185) Antibody

Description

Rabbit polyclonal antibody to SAPK (phospho Tyr185).

Specificity

This antibody detects endogenous levels of SAPK/JNK only when phosphorylated at Tyr185.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:20000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185 (amino acids 151-200).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

48kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-SAPK (phospho Tyr185) Antibody (A94409)

Western blot analysis of lysates from HepG2 cells treated with nocodazole 1µg/ml 16h using Anti-SAPK (phospho Tyr185) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Immunohistochemistry - Anti-SAPK (phospho Tyr185) Antibody (A94409)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-SAPK (phospho Tyr185) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-SAPK (phospho Tyr185) Antibody (A94409)

Western blot analysis of various cells using Anti-SAPK (phospho Tyr185) Antibody.

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Immunohistochemistry - Anti-SAPK (phospho Tyr185) Antibody (A94409)

Immunohistochemical analysis of paraffin-embedded human uterus tissue using Anti-SAPK (phospho Tyr185) Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.