Anti-Sarcomeric Alpha Actinin Antibody (A14449)

Western blot analysis of extracts of various cell lines, using Anti-Sarcomeric Alpha Actinin Antibody (A14449) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of HeLa cells using Anti-Sarcomeric Alpha Actinin Antibody (A14449) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).