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Anti-SARS-CoV-2 3CLPro Antibody (A307289)

Western Blot - Anti-SARS-CoV-2 3CLPro Antibody (A307289) - Antibodies.com Immunofluorescence - Anti-SARS-CoV-2 3CLPro Antibody (A307289) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to SARS-CoV-2 3CLPro for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-SARS-CoV-2 3CLPro Antibody
Description
Rabbit polyclonal antibody to SARS-CoV-2 3CLPro.
Applications
WB, ICC/IF
Dilutions
WB: 1:2,000-1:6,000, ICC/IF: 1:50-1:200
Reactivity
SARS-CoV-2
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of coronavirus 3CLpro (YP_009725295.1).
Sequence
MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
30 - 35 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-SARS-CoV-2 3CLPro Antibody (A307289) - Antibodies.comEnlarge Image

Western Blot - Anti-SARS-CoV-2 3CLPro Antibody (A307289)

Western blot analysis of extracts of 293T cells, using Anti-SARS-CoV-2 3CLPro Antibody (A307289) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-SARS-CoV-2 3CLPro Antibody (A307289) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-SARS-CoV-2 3CLPro Antibody (A307289)

Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 3CLpro fusion protein (top left) and untreated 293T cells (top right) using Anti-SARS-CoV-2 3CLPro Antibody (A307289) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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