Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody [2G1] (A270550)

Left Panel: HEK293 cells were transfected with DNA encoding the SARS-CoV-2 Spike Glycoprotein RBD for ACE2 which had been inserted into pCI-Neo-Mod or pCI-Neo-GFP eukaryotic expression vectors, such that the cells express either the insert only or the insert fused with GFP. The lanes contain: [Lane 1] protein standard, [Lane 2] a crude homogenate of untransfected control cells, [Lane 3] a homogenate of cells expressing SARS-CoV-2 Spike Glycoprotein RBD, and [Lane 4] a homogenate of cells expressing GFP-SARS-CoV-2 Spike Glycoprotein RBD fusion. Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody [2G1] (A270550), at a dilution of 1:3,000, in green, produces clean and specific staining of bands of the expected size. The blot was co-stained with Anti-HSP60 Antibody (A85437), at a dilution of 1:5,000, in red. Right Panel: Blot of full-length recombinant SARS-CoV-2 Spike Glycoprotein expressed in HEK293 cells; [Lanes 1] protein standard, [Lane 6] shows a loading of 1 µg, and [Lane 7] shows a loading of 100 ng. On longer exposure of the blot the antibody could readily detect 10ng of the S-protein.

Immunoflourescent analysis of HEK293 cells transfected with DNA encoding the S-protein segment in SARS-CoV-2 Spike Glycoprotein RBD which was inserted in the pCI-Neo-Mod expression vector. Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody [2G1] (A270550), in green, produces clean and specific staining of transfected cells. The nuclei of transfected and untransfected cells are shown in blue with DAPI DNA stain.

Immunofluorescent analysis of HEK293 cells transfected with a SARS-CoV-2 Spike Glycoprotein RBD for ACE2 construct under high magnification, stained with Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody [2G1] (A270550), at a dilution of 1:1,000, in green, and co-stained with Anti-HSP60 Antibody (A85437), at a dilution of 1:2,000, in red. The nuclear DNA is visualised in blue using Hoechst staining. Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody [2G1] (A270550) reveals overexpression of SARS-CoV-2 Spike Glycoprotein RBD presumably in lysosomes only in transfected cells, while the Anti-HSP60 Antibody (A85437) labels mitochondria in all cells.

Immunofluorescent analysis of transfected HEK293 cells with the SARS-CoV2-bd-construct under high magnification stained with mouse mAb to Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody [2G1] (A270550) at a dilution of 1:1,000 (green). Cells were costained with Anti-HSP60 Antibody (A85437) at a dilution of 1:2,000 (red). Nuclei were stained with Hoechst (blue). Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody [2G1] (A270550) reveals overexpression of SARS-CoV2-bd protein presumanbly in lysosomes only in transfected cells, while the HSP60 antibody labels mitochondria in all cells.