Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody (A306346)

Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike RBD Protein, using Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody (A306346) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody (A306346) can bind immobilized recombinant SARS-COV-2 Spike RBD protein at 1 µg/ml (100 µl/well) with a linear range of 0.78-50ng/ml.

Immunofluorescence analysis of 293T-RBD and 293T cells using Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody (A306346) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody (A306346). This Western blot was performed on the immunoprecipitate using Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody (A306346) at a dilution of 1:3000.