Recombinant rabbit monoclonal [DM26] antibody to SARS-CoV-2 Spike Glycoprotein RBD.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
SARS-CoV-2
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM26
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Competition flow cytometry assay demonstrating Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody [DM26] - Azide free (A318667) blockade of Recombinant SARS-CoV-2 Spike Glycoprotein RBD Protein (Fc Tag) (A318237) (1 µg/ml) binding to Expi 293 cell line transfected with human ACE2. IC50 = 1717 µg/ml. The Y-axis represents the geometric mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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