Western blot analysis of extracts of various cell lines, using Anti-SEC61B Antibody (A307609) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-SEC61B Antibody (A307609) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-SEC61B Antibody (A307609) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-SEC61B Antibody (A307609) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Proteins predicted to interact with SEC61B
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.