Western Blot - Anti-Selenium Binding Protein 1/SBP Antibody (A13390)
Western blot analysis of extracts of various cell lines, using Anti-Selenium Binding Protein 1/SBP Antibody (A13390) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit.
Western Blot - Anti-Selenium Binding Protein 1/SBP Antibody (A13390)
Western blot analysis of extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells, using Anti-Selenium Binding Protein 1/SBP Antibody (A13390) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.