Anti-Selenophosphate synthetase 1 Antibody (A15234)

Name: Anti-Selenophosphate synthetase 1 Antibody
Brand: Antibodies.com
SKU: A15234

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This product is discontinued

Anti-Selenophosphate synthetase 1 Antibody (A15234) has been discontinued and is no longer available.

View all Selenophosphate synthetase 1 Antibodies.

Specifications

Name

Anti-Selenophosphate synthetase 1 Antibody

Description

Rabbit polyclonal antibody to Selenophosphate synthetase 1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:10-1:100

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-392 of human SEPHS1 (NP_036379.2).

Sequence

MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

SELD, Selenide, water dikinase 1, Selenium donor protein 1, Selenophosphate synthase 1, SEPHS1, SPS, SPS1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Selenophosphate synthetase 1 Antibody (A15234)

Western blot analysis of extracts of various cell lines, using Anti-Selenophosphate synthetase 1 Antibody (A15234) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Selenophosphate synthetase 1 Antibody (A15234)

Immunohistochemistry analysis of paraffin-embedded human breast tissue using Anti-Selenophosphate synthetase 1 Antibody (A15234) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Selenophosphate synthetase 1 Antibody (A15234)

Immunohistochemistry analysis of paraffin-embedded human leiomyoma of uterus using Anti-Selenophosphate synthetase 1 Antibody (A15234) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.