Anti-Serum Amyloid P / SAP Antibody [AB18-4E2] (A279627)

Anti-Serum Amyloid P / SAP Antibody [AB18-4E2]

Mouse monoclonal [AB18-4E2] antibody to Serum Amyloid P / SAP.

This antibody recognises Serum Amyloid P-component (SAP) also known as 9.5S alpha-1-glycoprotein, a member of the pentraxin family of proteins. It is synthesized by hepatocytes and secreted into the blood. In humans the levels of SAP range between 20-50 µg/ml. SAP is able to bind to a variety of molecules including apoptotic debris, bacterial polysaccharides, amyloid deposits and bacterial toxins. Phagocytic cells such as monocytes and macrophages then bind the SAP and engulf the material bound to SAP as part of debris clearance. The purified Mouse anti Human SAP antibody, clone AB18-4E2 (MCA62648GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human SAP antibody, clone AB12-5A1 (MCA6269B) as the detection antibody.

ELISA: 1 µg/ml

Human

Native SAP purified from human serum.

Mouse

Monoclonal

AB18-4E2

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human SAP antibody, clone AB18-4E2, recognizes Serum Amyloid P-component (SAP) also known as 9.5S alpha-1-glycoprotein, a member of the pentraxin family of proteins. It is synthesized by hepatocytes and secreted into the blood. In humans the levels of SAP range between 20-50 µg/ml (Pilling & Gomer 2018). SAP is able to bind to a variety of molecules including apoptotic debris, bacterial polysaccharides, amyloid deposits and bacterial toxins. Phagocytic cells such as monocytes and macrophages then bind the SAP and engulf the material bound to SAP as part of debris clearance (Pilling & Gomer 2018).The purified Mouse anti Human SAP antibody, clone AB18-4E2 (MCA62648GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human SAP antibody, clone AB12-5A1 (MCA6269B) as the detection antibody.