Western blot analysis of Jurkat, using Anti-SETD3 Antibody (A307360) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 45s.
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-SETD3 Antibody (A307360). This Western blot was performed on the immunoprecipitate using Anti-SETD3 Antibody (A307360) at a dilution of 1:1000.
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