Anti-SFRP4 Antibody [ARC0923] (A305346)

Name: Anti-SFRP4 Antibody [ARC0923]
Brand: Antibodies.com
SKU: A305346
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0923] antibody to SFRP4 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-SFRP4 Antibody [ARC0923]

Description

Rabbit monoclonal [ARC0923] antibody to SFRP4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-300 of human SFRP4 (Q6FHJ7).

Sequence

AKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0923

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Frizzled protein, human endometrium, FrpHE, Secreted frizzled-related protein 4, sFRP-4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-SFRP4 Antibody [ARC0923] (A305346)

Western blot analysis of extracts of various cell lines, using Anti-SFRP4 Antibody [ARC0923] (A305346) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-SFRP4 Antibody [ARC0923] (A305346)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SFRP4 Antibody [ARC0923] (A305346) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-SFRP4 Antibody [ARC0923] (A305346)

Immunofluorescence analysis of NIH-3T3 cells using Anti-SFRP4 Antibody [ARC0923] (A305346) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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