This antibody recognises endophilin-A1, also known as SH3 domain protein 2A, SH3 domain protein 2A and EEN-B1. Endophilins belong to the family of Bin/Amphiphysin/Rvs (BAR) proteins and are composed of an N-terminal BAR domain and a C-terminal SH3 domain. Endophilin-A1 is essential for synaptic vesicle endocytosis. Its interaction with ataxin-2 and huntingtin is important for plastin-associated cellular pathways. Endophilin-A1 is also involved in regulation of blood-brain barrier permeability through the EGFR-JNK signaling pathway.
Applications
WB
Dilutions
WB: 1:500 - 1:2,000
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human endophilin-A1.
Approximately 42 kDa in various cell lysates under reducing conditions
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.02% Sodium Azide.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti endophilin-A1 antibody recognizes endophilin-A1, also known as SH3 domain protein 2A, SH3 domain protein 2A and EEN-B1. Endophilins belong to the family of Bin/Amphiphysin/Rvs (BAR) proteins and are composed of an N-terminal BAR domain and a C-terminal SH3 domain (Safari and Suetsugu 2012). Endophilin-A1 is essential for synaptic vesicle endocytosis (Llobet et al. 2011). Its interaction with ataxin-2 and huntingtin is important for plastin-associated cellular pathways (Ralser et al. 2005). Endophilin-A1 is also involved in regulation of blood-brain barrier permeability through the EGFR-JNK signaling pathway (Chen et al. 2015).
Synonyms
CNSA2, EEN-B1, Endophilin-1, Endophilin-A1, SH3 domain protein 2A, SH3 domain-containing GRB2-like protein 2, SH3D2A
