Anti-Sheep IgE Antibody [1E7] (A278870)

Anti-Sheep IgE Antibody [1E7]

Mouse monoclonal [1E7] antibody to Sheep IgE.

This antibody recognises ovine Immunoglobulin E (IgE) and does not cross react with ovine IgM, IgA, IgG1 or IgG2.IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7 demonstrates a single major band of approximately 80 kDa under reducing conditions, and a band at approximately 200 kDa is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule. IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. Mouse anti Sheep IgE, clone 1E7 has been used to investigate parasite nematode infections of sheep by Haemonchus contortus (Vervelde et al. 1997 and Kooyman et al. 1997). Clone 1E7 has also been reported to recognize bovine IgE, and, as such, it is useful to detect response to binfection in cattle by nematode worms such as Dictyocaulus viviparus (Bricarello et al. 2007 and Kooyman et al. 2002).In addition to this specific anti IgE monoclonal antibody, monoclonal antibodies specific to both bovine and ovine, IgA, IgM and IgG subclasses are available from Bio-Rad.

WB, ELISA, IF, IHC-Fr

ELISA: 1:500 - 1:2,000

Sheep, Goat

This antibody does not cross react with Horse or Human

Recombinant sheep IgE1-2 expressed in E.coli.

Mouse

Monoclonal

1E7

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

500 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Sheep IgE, clone 1E7, recognizes ovine Immunoglobulin E (IgE) and does not cross react with ovine IgM, IgA, IgG1 or IgG2.IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7 demonstrates a single major band of approximately 80 kDa under reducing conditions, and a band at approximately 200 kDa is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule (Kooyman et al. 1997).IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. Mouse anti Sheep IgE, clone 1E7 has been used to investigate parasite nematode infections of sheep by Haemonchus contortus (Vervelde et al. 1997 and Kooyman et al. 1997). Clone 1E7 has also been reported to recognize bovine IgE, and, as such, it is useful to detect response to binfection in cattle by nematode worms such as Dictyocaulus viviparus (Bricarello et al. 2007 and Kooyman et al. 2002).In addition to this specific anti IgE monoclonal antibody, monoclonal antibodies specific to both bovine and ovine, IgA, IgM and IgG subclasses are available from Bio-Rad.