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Anti-SHMT2/SHMT Antibody (A13383)

Western Blot - Anti-SHMT2/SHMT Antibody (A13383) - Antibodies.com Western Blot - Anti-SHMT2/SHMT Antibody (A13383) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit polyclonal antibody to SHMT2/SHMT for WB, IHC, ICC/IF and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-SHMT2/SHMT Antibody
Description
Rabbit polyclonal antibody to SHMT2/SHMT.
Applications
WB, IHC, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2).
Sequence
PSPFKHADIVTTTTHKTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAFPMPGFDEH
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
56 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-SHMT2/SHMT Antibody (A13383) - Antibodies.comEnlarge Image

Western Blot - Anti-SHMT2/SHMT Antibody (A13383)

Western blot analysis of extracts of various cell lines, using Anti-SHMT2/SHMT Antibody (A13383) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-SHMT2/SHMT Antibody (A13383) - Antibodies.comEnlarge Image

Western Blot - Anti-SHMT2/SHMT Antibody (A13383)

Western blot analysis of extracts from normal (control) and SHMT2 Rabbit polyclonal antibody knockout (KO) HeLa cells, using Anti-SHMT2/SHMT Antibody (A13383) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
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