Anti-Sialoadhesin / CD169 Antibody [3B11/11] (FITC) (A279285)

Anti-Sialoadhesin / CD169 Antibody [3B11/11] (FITC)

Mouse monoclonal [3B11/11] antibody to Sialoadhesin / CD169 (FITC).

This antibody recognises porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor. CD169 has subsequently been identified in rat , human and pig. Mouse anti Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages. Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer’s patches.Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor and as a targeted receptor for the delivery of toxins and antigens. Mouse anti pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revilla et al 2009).

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to 1x10⁶ cells in 100µl

Porcine

Porcine alveolar macrophages.

Mouse

Monoclonal

3B11/11

Protein A affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Pig CD169, clone 3B11/11 recognizes porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor (Crocker et al.1986). CD169 has subsequently been identified in rat (van den Berg et al. 1992), human (Mucklow et al. 1995) and pig (Vanderheijden et al. 2003) . Mouse anti Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullido et al. 1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer’s patches.Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputte et al. 2006) and as a targeted receptor for the delivery of toxins and antigens (Delputte et al. 2011) .Mouse anti pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revilla et al 2009).

This product is for research use only. It is not intended for diagnostic or therapeutic use.