Mouse monoclonal [7-239] antibody to Sialoadhesin/CD169 (APC).
Specificity
This antibody recognizes an extracellular epitope of CD169, a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human rhinovirus 14 infected monocyte-derived dendritic cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
7-239
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
210 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human TNF alpha and IFN gamma stimulated peripheral blood mononuclear cells stained with Anti-CD169 Antibody [7-239] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
Flow cytometric analysis of human TNF alpha and IFN gamma stimulated peripheral blood mononuclear cells stained using Anti-CD169 Antibody [7-239] (APC) (10µl reagent per milion cells in 100µl of cell suspension) and Anti-CD11c Antibody [BU15] (FITC) (20µl reagent per 100µl of peripheral whole blood).
Separation of human CD169 positive CD11c positive cells (red-filled) from CD169 negative CD11c negative cells (black-dashed) in flow cytometry analysis of human TNF alpha and IFN gamma stimulated peripheral blood mononuclear cells using Anti-CD169 Antibody [7-239] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
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