Anti-Siglec 10 Antibody [5G6] (PE) (A279449)

Anti-Siglec 10 Antibody [5G6] (PE)

Mouse monoclonal [5G6] antibody to Siglec 10 (PE).

This antibody recognises human Siglec-10 (Sialic acid-binding Ig-like lectin 10), a putative adhesion molecule and member of the Ig superfamily, expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16+CD56- natural killer (NK) cells. Structurally, Siglec-10 is most similar to the CD33-related group of Siglecs, and preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid. Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking,. This interaction between Siglec-10 and VAP-1, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment. Mouse anti Human Siglec-10 antibody, clone 5G6 does not cross-react with Siglecs 3, 5, 7, 8 and 9.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells.

Human

Recombinant human Siglec-10, fused with the Fc region of human IgG.

Mouse

Monoclonal

5G6

IgG1

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human Siglec-10 antibody, clone 5G6 recognises human Siglec-10 (Sialic acid-binding Ig-like lectin 10), a putative adhesion molecule and member of the Ig superfamily, expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16+CD56- natural killer (NK) cells. Structurally, Siglec-10 is most similar to the CD33-related group of Siglecs, and preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid. Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking, (Kivi et al. 2009). This interaction between Siglec-10 and VAP-1, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment. Mouse anti Human Siglec-10 antibody, clone 5G6 does not cross-react with Siglecs 3, 5, 7, 8 and 9 (Munday et al. 2001).

This product is for research use only. It is not intended for diagnostic or therapeutic use.