This antibody recognizes an extracellular epitope of SIGLEC8, an eosinophil marker, expressed in areas including lymph nodes and spleen.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Extracellular domain of human SIGLEC8 fused with Fc fragment of human IgG1.
Host
Mouse
Clonality
Monoclonal
Clone ID
7C9
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
SAF-2, SAF2, Sialic acid-binding Ig-like lectin 8, Sialoadhesin family member 2, Siglec-8
Separation of human SIGLEC8 positive eosinophil granulocytes (red-filled) from eosinophil granulocytes (black-dashed) stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 1,7 µg/ml, same as SIGLEC8 PE concentration, black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling SIGLEC8 with Anti-SIGLEC8 Antibody [7C9] (PE) (A320791), (10 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with SIGLEC8
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-SIGLEC8 Antibody [7C9] (PE) (A320791)](https://cdn.antibodies.com/image/catalog/320/A320791_1.jpg?profile=product_top)
![Flow Cytometry - Anti-SIGLEC8 Antibody [7C9] (PE) (A320791)](https://cdn.antibodies.com/image/catalog/320/A320791_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-SIGLEC8 Antibody [7C9] (PE) (A320791)](https://cdn.antibodies.com/image/catalog/320/A320791_1.jpg?profile=product_image)