Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
hSIRT6, NAD-dependent protein deacetylase sirtuin-6, NAD-dependent protein deacylase sirtuin-6, Protein mono-ADP-ribosyltransferase sirtuin-6, Regulatory protein SIR2 homolog 6, SIR2-like protein 6, SIR2L6
Western blot analysis of extracts of various cell lines, using Anti-SIRT6 Antibody (A307885) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SIRT6 Antibody (A307885) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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