Anti-SLAMF7/CS1 Antibody [DM9] - BSA and Azide free
Description
Recombinant rabbit monoclonal [DM9] antibody to SLAMF7/CS1.
Applications
ELISA, Flow Cytometry
Dilutions
Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM9
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Expi 293 cell line was transfected with irrelevant protein (A) and human CS1 (B) were surface stained with Anti-SLAMF7/CS1 Antibody [DM9] - BSA and Azide free (A318676) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Flow cytometry data of serially titrated Anti-SLAMF7/CS1 Antibody [DM9] - BSA and Azide free (A318676) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit Anti-CS1 Monoclonal Antibody clones by titration of different concentrations onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Flow cytometry analysis of antigen binding of Anti-SLAMF7/CS1 Antibody [DM9] - BSA and Azide free (A318676). (A) Anti-SLAMF7/CS1 Antibody [DM9] - BSA and Azide free (A318676) does not bind to 293T cells that do not express CS1. (B) A clear peak shift of Anti-SLAMF7/CS1 Antibody [DM9] - BSA and Azide free (A318676) was seen compared to the control when incubated with CS1-expressing MM.1S cells, indicating strong binding of Anti-SLAMF7/CS1 Antibody [DM9] - BSA and Azide free (A318676) to CS1. Antibodies were incubated at 2 µg/ml.
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![Phylogenetic Analysis - Anti-SLAMF7/CS1 Antibody [DM9] - BSA and Azide free (A318676) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318676_4.png?profile=product_top_thumb)
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![Phylogenetic Analysis - Anti-SLAMF7/CS1 Antibody [DM9] - BSA and Azide free (A318676) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318676_4.png?profile=product_image)
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