Anti-Smac/Diablo Antibody [ARC1340] (A305474)

Western blot analysis of extracts of various cell lines, using Anti-Smac/Diablo Antibody [ARC1340] (A305474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Smac/Diablo Antibody [ARC1340] (A305474) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Confocal imaging of U-2OS cells using Anti-Smac/Diablo Antibody [ARC1340] (A305474), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.