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Anti-Smad1 (phospho Ser463 + Ser465) + SMAD5 (phospho Ser463 + Ser465) + SMAD9 (phospho Ser465 + Ser467) Antibody (A90723)

Western Blot - Anti-Smad1 (phospho Ser463 + Ser465) + SMAD5 (phospho Ser463 + Ser465) + SMAD9 (phospho Ser465 + Ser467) Antibody (A90723) - Antibodies.com
This product is discontinued

Anti-Smad1 (phospho Ser463 + Ser465) + SMAD5 (phospho Ser463 + Ser465) + SMAD9 (phospho Ser465 + Ser467) Antibody (A90723) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-Smad1 (phospho Ser463 + Ser465) + SMAD5 (phospho Ser463 + Ser465) + SMAD9 (phospho Ser465 + Ser467) Antibody
Description
Rabbit polyclonal antibody to Smad1 (phospho Ser463 + Ser465) + SMAD5 (phospho Ser463 + Ser465) + SMAD9 (phospho Ser465 + Ser467).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around S463 & S465 of human Smad1SMAD1 (NP_005891.1).
Sequence
SSVS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Smad1 (phospho Ser463 + Ser465) + SMAD5 (phospho Ser463 + Ser465) + SMAD9 (phospho Ser465 + Ser467) Antibody (A90723) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad1 (phospho Ser463 + Ser465) + SMAD5 (phospho Ser463 + Ser465) + SMAD9 (phospho Ser465 + Ser467) Antibody (A90723)

Western blot analysis of extracts of HeLa cells, using Anti-Smad1 (phospho Ser463 + Ser465) + SMAD5 (phospho Ser463 + Ser465) + SMAD9 (phospho Ser465 + Ser467) Antibody (A90723) at 1:1,000 dilution. HeLa cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.