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Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A93937)

Western Blot - Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A1001) - Antibodies.com Western Blot - Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A1001) - Antibodies.com Western Blot - Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A1001) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to Smad2 + Smad3 (phospho Thr8) for WB and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-Smad2 + Smad3 (phospho Thr8) Antibody
Description
Rabbit polyclonal antibody to Smad2 + Smad3 (phospho Thr8).
Specificity
This antibody detects endogenous levels of Smad2/3 only when phosphorylated at Thr8.
Applications
WB, ELISA
Dilutions
IHC: 1:50-1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Smad2/3 around the phosphorylation site of Thr8 (amino acids 1-50).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
48kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A1001) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A93937)

Western blot analysis of lysates from RAW264.7 cells using Anti-Smad2 + Smad3 (phospho Thr8) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Western Blot - Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A1001) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A93937)

Western blot analysis of various cells using Anti-Smad2 + Smad3 (phospho Thr8) Antibody.
Western Blot - Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A1001) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad2 + Smad3 (phospho Thr8) Antibody (A93937)

Western blot analysis of VEC cells using Anti-Smad2 + Smad3 (phospho Thr8) Antibody.
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