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Anti-Smad3 (phospho Ser204) Antibody (A93814)

Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A1004) - Antibodies.com Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A1004) - Antibodies.com Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A1004) - Antibodies.com Immunohistochemistry - Anti-Smad3 (phospho Ser204) Antibody (A1004) - Antibodies.com
This product is discontinued

Anti-Smad3 (phospho Ser204) Antibody (A93814) has been discontinued and is no longer available.

View all Smad3 Antibodies.

  • Specifications

Name
Anti-Smad3 (phospho Ser204) Antibody
Description
Rabbit polyclonal antibody to Smad3 (phospho Ser204).
Specificity
This antibody detects endogenous levels of Smad3 only when phosphorylated at Ser204.
Applications
WB, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Smad3 around the phosphorylation site of Ser204 (amino acids 170-219).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Concentration
1mg/ml
Molecular Weight
48kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A1004) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A93814)

Western blot analysis of lysates from NIH/3T3 cells treated with Serum 20% 15' using Anti-Smad3 (phospho Ser204) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A1004) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A93814)

Western blot analysis of various cells using Anti-Smad3 (phospho Ser204) Antibody.
Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A1004) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A93814)

Western blot analysis of MCF 7 cells using Anti-Smad3 (phospho Ser204) Antibody.
Immunohistochemistry - Anti-Smad3 (phospho Ser204) Antibody (A1004) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Smad3 (phospho Ser204) Antibody (A93814)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-Smad3 (phospho Ser204) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Proteins predicted to interact with Smad3

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score