Anti-SMAD6 Antibody (A92311)

Name: Anti-SMAD6 Antibody
Brand: Antibodies.com
SKU: A92311

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This product is discontinued

Anti-SMAD6 Antibody (A92311) has been discontinued and is no longer available.

View all SMAD6 Antibodies.

Specifications

Name

Anti-SMAD6 Antibody

Description

Rabbit polyclonal antibody to SMAD6.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 257-496 of human Smad6 (NP_005576.3).

Sequence

PTVCCNPYHFSRLCGPESPPPPYSRLSPRDEYKPLDLSDSTLSYTETEATNSLITAPGEFSDASMSPDATKPSHWCSVAYWEHRTRVGRLYAVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVWAYNRGEHPIFVNSPTLDAPGGRALVVRKVPPGYSIKVFDFERSGLQHAPEPDAADGPYDPNSVRISFAKGWGPCYSRQFITSCPCWLEILLNNPR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

53 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hSMAD6, MAD homolog 6, MADH6, Mothers against decapentaplegic homolog 6, Mothers against DPP homolog 6, SMAD 6, SMAD family member 6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-SMAD6 Antibody (A92311)

Western blot analysis of extracts of various cell lines, using Anti-SMAD6 Antibody (A92311) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 15s.

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Immunofluorescence - Anti-SMAD6 Antibody (A92311)

Immunofluorescence analysis of NIH-3T3 cells using Anti-SMAD6 Antibody (A92311) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).