Anti-SMYD4 Antibody (A91412)

Name: Anti-SMYD4 Antibody
Brand: Antibodies.com
SKU: A91412
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to SMYD4 for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-SMYD4 Antibody

Description

Rabbit polyclonal antibody to SMYD4.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:20-1:50

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).

Sequence

MDLPVDEWKSYLLQKWASLPTSVQVTISTAETLRDIFLHSSSLLQPEDELFLKRLSKGYLVGKDSDAPLFYREEGNKKFQEKDYTGAAVLYSKGVSHSRPNTEDMSLCHANRSAALFHLGQYETCLKDINRAQTHGYPERLQPKIMLRKAECLVALGRLQEASQTISDLERNFTATPALADVLPQTLQRNLHRLKMKMQEKDSLTESFPAALAKTLEDAALREENEQLSNASSSIGLCVDPLKGRCLVATKDILPGELLV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

KIAA1936, SET and MYND domain-containing protein 4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-SMYD4 Antibody (A91412)

Western blot analysis of extracts of various cell lines, using Anti-SMYD4 Antibody (A91412) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunofluorescence - Anti-SMYD4 Antibody (A91412)

Immunofluorescence analysis of MCF-7 cells using Anti-SMYD4 Antibody (A91412). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-SMYD4 Antibody (A91412)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 1µg of Anti-SMYD4 Antibody (A91412). This Western blot was performed on the immunoprecipitate using Anti-SMYD4 Antibody (A91412) at a dilution of 1:500. Detection was with a ECL Enhanced Kit. Exposure time: 20s.

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