Recombinant rabbit monoclonal [1G1] antibody to SN38.
Applications
ELISA
Dilutions
ELISA: 1:5,000-10,000
Host
Rabbit
Clonality
Monoclonal
Clone ID
1G1
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%-8% Trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Anti-SN38 Antibody [1G1] - BSA and Azide free (A324897)
ELISA plates were pre-coated with IgG-SN38 at 0.2µg/per well. Serial diluted Anti-SN38 Antibody [1G1] - BSA and Azide free (A324897) solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-SN38 Antibody [1G1] - BSA and Azide free (A324897) binding with IgG-SN38 is 11.39ng/ml.
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