Anti-Sodium Potassium ATPase Antibody (A12666)

Name: Anti-Sodium Potassium ATPase Antibody
Brand: Antibodies.com
SKU: A12666

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This product is discontinued

Anti-Sodium Potassium ATPase Antibody (A12666) has been discontinued and is no longer available.

View all Sodium Potassium ATPase Antibodies.

Specifications

Name

Anti-Sodium Potassium ATPase Antibody

Description

Rabbit polyclonal antibody to Sodium Potassium ATPase.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human ATP1A (NP_000692.2).

Sequence

RVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

113 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATP1A1, ATP1A4, ATP1AL2, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase alpha-4 subunit, Sodium pump subunit alpha-1, Sodium pump subunit alpha-4, Sodium/potassium-transporting ATPase subunit alpha-1, Sodium/potassium-transporting ATPase subunit alpha-4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Sodium Potassium ATPase Antibody (A12666)

Western blot analysis of extracts of various cell lines, using Anti-Sodium Potassium ATPase Antibody (A12666) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-Sodium Potassium ATPase Antibody (A12666)

Western blot analysis of extracts of HeLa cells, using Anti-Sodium Potassium ATPase Antibody (A12666) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-Sodium Potassium ATPase Antibody (A12666)

Immunohistochemistry analysis of paraffin-embedded human prostate using Anti-Sodium Potassium ATPase Antibody (A12666) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Sodium Potassium ATPase Antibody (A12666)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-Sodium Potassium ATPase Antibody (A12666) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.