Anti-SPAG1 Antibody (A48154)

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This product is discontinued

Anti-SPAG1 Antibody (A48154) has been discontinued and is no longer available.

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Specifications

Name

Anti-SPAG1 Antibody

Description

Rabbit polyclonal antibody to SPAG1

Specificity

The antibody detects endogenous level of total SPAG1 protein.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:1000-1:5000 Western blotting: 1:500-1:2000 Immunohistochemistry: 1:25-1:100

Reactivity

Human

Immunogen

Synthetic peptide corresponding to a region derived from 760-774 amino acids of Human sperm associated antigen 1

Host

Rabbit

Clonality

Polyclonal

Conjugate

Unconjugated

Predicted MW

104kd

Formulation

Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.3, containing 150 mM NaCl, 0.05% sodium azide, and 50% glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

HSD-3.8, Infertility-related sperm protein Spag-1, Sperm-associated antigen 1

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Anti-SPAG1 Antibody (A48154)

Gel: 8% SDS-PAGE Lysate: 40 µg Human colon tissue lysate Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes

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Anti-SPAG1 Antibody (A48154)

The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using 31270 (SPAG1 Antibody) at dilution 1/25, on the right is treated with the synthetic peptide.