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Anti-SPARC Antibody (A13550)

Western Blot - Anti-SPARC Antibody (A1615) - Antibodies.com Immunohistochemistry - Anti-SPARC Antibody (A1615) - Antibodies.com Immunohistochemistry - Anti-SPARC Antibody (A1615) - Antibodies.com Immunohistochemistry - Anti-SPARC Antibody (A1615) - Antibodies.com Immunohistochemistry - Anti-SPARC Antibody (A1615) - Antibodies.com Immunohistochemistry - Anti-SPARC Antibody (A1615) - Antibodies.com Immunohistochemistry - Anti-SPARC Antibody (A1615) - Antibodies.com
This product is discontinued

Anti-SPARC Antibody (A13550) has been discontinued and is no longer available.

View all SPARC Antibodies.

  • Specifications

Name
Anti-SPARC Antibody
Description
Rabbit polyclonal antibody to SPARC.
Applications
WB, IHC, IF
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1).
Sequence
APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
35kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Basement-membrane protein 40, BM-40, ON, Osteonectin, Secreted protein acidic and rich in cysteine
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (7)

Western Blot - Anti-SPARC Antibody (A1615) - Antibodies.comEnlarge Image

Western Blot - Anti-SPARC Antibody (A13550)

Western blot analysis of extracts of various cell lines, using Anti-SPARC Antibody (A1615) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST.
Immunohistochemistry - Anti-SPARC Antibody (A1615) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-SPARC Antibody (A13550)

Immunohistochemistry of paraffin-embedded rat Intestine using Anti-SPARC Antibody (A1615) at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-SPARC Antibody (A1615) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-SPARC Antibody (A13550)

Immunohistochemistry of paraffin-embedded human lung cancer using Anti-SPARC Antibody (A1615) at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-SPARC Antibody (A1615) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-SPARC Antibody (A13550)

Immunohistochemistry of paraffin-embedded human liver cancer using Anti-SPARC Antibody (A1615) at dilution of 1:100 (40x lens).

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Proteins predicted to interact with SPARC

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.1% Relevancy Score
99.1% Relevancy Score
98.4% Relevancy Score
96.9% Relevancy Score
96.8% Relevancy Score
96.5% Relevancy Score
95.9% Relevancy Score
95.4% Relevancy Score
93.1% Relevancy Score