Recombinant Anti-SPARC Antibody [OSTN/8528R] (A315673)

Anti-SPARC Antibody [OSTN/8528R]

Recombinant rabbit monoclonal [OSTN/8528R] antibody to SPARC.

SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Human placenta, lymph node or spleen. JEG3 or A549 cell lysates.

IHC: 1-2 µg/ml

Human

Recombinant fragment (around Amino Acids 1-200) of human SPARC protein. The exact sequence is proprietary.

Rabbit

Monoclonal

OSTN/8528R

kappa

Protein A/G chromatography.

200 µg/ml

38 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Basement-membrane protein 40, BM-40, ON, Osteonectin, Secreted protein acidic and rich in cysteine