Spartin expression in HeLa cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Spartin Antibody (A84633) at 2µg/ml and detected by chemiluminescence.
Spartin expression in HeLa before (left) and after (right) introduction of siRNA analyzed by immunofluorescence. Cells were fixed in methanol. Staining was performed with Anti-Spartin Antibody (A84633) at 2µg/ml.
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