Anti-STAT1 (phospho Ser727) Antibody [ARC1544] (A308199)

Western blot analysis of extracts of various cell lines, using Anti-STAT1 (phospho Ser727) Antibody [ARC1544] (A308199) at 1:1,000 dilution. Both HeLa cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1min.

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-STAT1 (phospho Ser727) Antibody [ARC1544] (A308199) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-STAT1 (phospho Ser727) Antibody [ARC1544] (A308199) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.