Rabbit polyclonal antibody to Steroidogenic Factor 1/SF-1.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Steroidogenic factor-1 (SF-1/Steroidogenic factor-1 (SF-1/NR5A1)) (NP_004950.2).
Western Blot - Anti-Steroidogenic Factor 1/SF-1 Antibody (A90342)
Western blot analysis of extracts of Mouse testis, using Anti-Steroidogenic Factor 1/SF-1 Antibody (A90342) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-Steroidogenic Factor 1/SF-1 Antibody (A90342)
Western blot analysis of extracts of various cell lines, using Anti-Steroidogenic Factor 1/SF-1 Antibody (A90342) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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