Anti-STRADB Antibody (A82463)

STRADB expression in Human Heart lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-STRADB Antibody (A82463) at 1µg/ml and detected by chemiluminescence.

STRADB expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress STRADB and incubated with Anti-STRADB Antibody (A82463). Control: Mock-transfected HEK293 cell lysate incubated with Anti-STRADB Antibody (A82463) (first lane).

STRADB expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-STRADB Antibody (A82463) at 3.8µg/ml and revealed with alkaline phosphatase (AP).

STRADB expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-STRADB Antibody (A82463) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.