Anti-Sulfatase 2 / SULF2 Antibody [2B4] (A279183)

Anti-Sulfatase 2 / SULF2 Antibody [2B4]

Mouse monoclonal [2B4] antibody to Sulfatase 2 / SULF2.

This antibody recognises an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Sulf-2 is linked with several cancers, including breast, hepatocellular, pancreatic and non-small-cell lung carcinomas. Mature human Sulf-2 is a heterodimer consisting of a 50 kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75 kDa N-terminal subunit which contains the catalytic centre. recognises Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse.

WB, IHC-P, ELISA

Human, Mouse

Recombinant human Sulf-2.

Mouse

Monoclonal

2B4

IgG1

Protein G affinity chromatography of tissue culture supernatant.

Approximately 50 kDa in Sulf-2 transfected HEK293 cell lysates. A band at 135 kDa (unprocessed protein), and a fragment at 37 kDa, may also be present.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Sulf-2 is linked with several cancers, including breast, hepatocellular, pancreatic and non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010). Mature human Sulf-2 is a heterodimer consisting of a 50 kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75 kDa N-terminal subunit which contains the catalytic centre. Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse.