Western Blot - Anti-SUN2 Antibody [ARC2311] (A308466)
Western blot analysis of extracts of various cell lines, using Anti-SUN2 Antibody [ARC2311] (A308466) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-SUN2 Antibody [ARC2311] (A308466) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-SUN2 Antibody [ARC2311] (A308466) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-SUN2 Antibody [ARC2311] (A308466) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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