Anti-Superoxide Dismutase 3 / EC-SOD Antibody (A304989)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~35 kDa SOD (EC) protein using Anti-Superoxide Dismutase 3 / EC-SOD Antibody (A304989) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: human Cervical cancer cell line (HeLa) lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~35 kDa. Other Band(s): 50 kDa.

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Superoxide Dismutase 3 / EC-SOD Antibody (A304989), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite.

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Superoxide Dismutase 3 / EC-SOD Antibody (A304989), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite.