This antibody recognises the porcine SWC5 cell surface antigen. SWC5 has a molecular weight of 180 kDa and is expressed only on subset of ?/d T cells. SWC5 and CD2 expression divides ?d T cells into three subpopulations: CD2-SWC5-, CD2-SWC5+ and CD2+SWC5- ?d T cells subsets. The vast majority of CD2-SWC5- and CD2-SWC5+ T cells are negative for CD8a and SLA-DR and positive for CD27. Only thymus and spleen has an extra CD2+SWC5+ population.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10
Reactivity
Porcine
Immunogen
Porcine T cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
b37c10
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.
General Notes
Mouse anti Pig SWC5 antibody, clone b37c10 recognizes the porcine SWC5 cell surface antigen. SWC5 has a molecular weight of 180 kDa and is expressed only on subset of ?/d T cells. SWC5 and CD2 expression divides ?d T cells into three subpopulations: CD2-SWC5-, CD2-SWC5+ and CD2+SWC5- ?d T cells subsets. The vast majority of CD2-SWC5- and CD2-SWC5+ T cells are negative for CD8a and SLA-DR and positive for CD27. Only thymus and spleen has an extra CD2+SWC5+ population.
Figure A. Anti-CD3 epsilon Antibody [PPT3] (FITC) (A281589) and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Anti-CD3 epsilon Antibody [PPT3] (FITC) (A281589) and Anti-SWC5 Antibody [b37c10] (PE) (A279766). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
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