Anti-T Lymphocytes Antibody [KEN-5] (A280817)

Anti-T Lymphocytes Antibody [KEN-5]

Mouse monoclonal [KEN-5] antibody to T Lymphocytes.

This antibody recognises rabbit (lapine) T-cells. Clone KEN-5 recognises >90% thymocytes and approximately 40-45% mesenteric lymph node cells and immunoprecipitates a ~67 kDa protein from rabbit thymocytes.In contrast to humans and mice where only a very small population of B-cells express the CD5 antigen, in rabbits it is widely expressed by peripheral blood B cells. However, the KEN-5 antibody, while originally reported as recognizing rabbit CD5 , does not bind to rabbit CD5 transfectants. Furthermore, clone KEN-5, unlike other known antibodies recognizing rabbit CD5 and anti human cross reactive CD5 antibodies, shows very limited binding to rabbit B-cells, which in adult rabbits express CD5 Clone KEN-5 may recognize a epitope that is dependant on glycosylation to maintain its structural configuration which may explain why this antibody does not recognize recombinant rabbit CD5 produced in insect cells which contain different glycans from those found in mammalian cells. KEN-5 may distinguish between different isoforms of CD5 in lapine T and B cells.

Flow Cytometry, ELISA, IHC-Fr, IP

Flow Cytometry: 1:50 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rabbit

This antibody does not cross react with Mouse

Rabbit Thymocytes.

Mouse

Monoclonal

KEN-5

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Rabbit T lymphocytes, clone KEN-5, recognizes rabbit (lapine) T-cells. Clone KEN-5 recognizes >90% thymocytes and approximately 40-45% mesenteric lymph node cells and immunoprecipitates a ~67 kDa protein from rabbit thymocytes.In contrast to humans and mice where only a very small population of B-cells express the CD5 antigen, in rabbits it is widely expressed by peripheral blood B cells. However, the KEN-5 antibody, while originally reported as recognizing rabbit CD5 (Kotani et al.1993), does not bind to rabbit CD5 transfectants. Furthermore, clone KEN-5, unlike other known antibodies recognizing rabbit CD5 and anti human cross reactive CD5 antibodies, shows very limited binding to rabbit B-cells, which in adult rabbits express CD5 (Raman & Knight 1992) Clone KEN-5 may recognize a epitope that is dependant on glycosylation to maintain its structural configuration which may explain why this antibody does not recognize recombinant rabbit CD5 produced in insect cells which contain different glycans from those found in mammalian cells. KEN-5 may distinguish between different isoforms of CD5 in lapine T and B cells (Pospisil et al. 2009).