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Anti-TAF1 Antibody (A389)

Identification of TAF1 protein in whole cell extract of HeLa cells by western blotting using anti-TAF1 antibody. Lane 1; Size marker proteins (kDa) Lane 2; HeLa cell whole extract (10 µg) Arrow indicates the position of TAF1 protein band Immunofluorescence staining of TAF1 protein in BHK cells with anti-TAF1 antibody. (a) Reacted with anti-TAF1 antibody (b) Reacted with anti-TAF1 antibody in the presence of the immunogen peptide. (c) The same field as (a), stained with Hoechst 33258. (d) The same field as (b), stained with Hoechst 33258. (e) The same field as (a) and (c), photographed through phase-contrast microscope (f) The same field as (b) and (d), photographed through phase-contrast microscope. Growing cells were fixed with 3% formaldehyde and permiabilized 1% Nonidet P-40. As the 2nd antibody, goat anti-rabbit IgG conjugated with rhodamine was used. TAF1 protein was detected in nucleus. Immunofluorescence staining of TAF1 protein in HeLa cells with anti-TAF1 antibody. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100. Anti-TAF1 antibody was used at 1/5,000 dilution. As a 2nd antibody, goat anti-rabbit IgG conjugated with Alex 488 was used at 1/1,000 dilution. Nucliei were stained with DAPI. The merged image was shown in the right panel. TAF1 protein was detected in nucleus.
$555
Product Datasheet

Rabbit polyclonal antibody to TAF1 for WB and IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-TAF1 Antibody
Description
Rabbit polyclonal antibody to TAF1.
Applications
WB, IF
Dilutions
WB: 1:1,000, IF: 1:1,000-1:5,000
Reactivity
Human, Hamster, Mouse, Rat
Immunogen
Synthetic peptide corresponding to human TFIID subunit 1 protein (a.a. 103-123), conjugated with KLH.
Sequence
STEDAVDYSDINEVAEDESRR-C
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Product Form
Liquid
Formulation
Supplied as antiserum with 0.5% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BA2R, CCG1, CCGS, Cell cycle gene 1 protein, p250, TAF(II)250, TAF2A, TAFII-250, TAFII250, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, Transcription initiation factor TFIID subunit 1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Identification of TAF1 protein in whole cell extract of HeLa cells by western blotting using anti-TAF1 antibody. Lane 1; Size marker proteins (kDa) Lane 2; HeLa cell whole extract (10 µg) Arrow indicates the position of TAF1 protein bandEnlarge Image

Anti-TAF1 Antibody (A389)

Anti-TAF1 Antibody
Immunofluorescence staining of TAF1 protein in BHK cells with anti-TAF1 antibody. (a) Reacted with anti-TAF1 antibody (b) Reacted with anti-TAF1 antibody in the presence of the immunogen peptide. (c) The same field as (a), stained with Hoechst 33258. (d) The same field as (b), stained with Hoechst 33258. (e) The same field as (a) and (c), photographed through phase-contrast microscope (f) The same field as (b) and (d), photographed through phase-contrast microscope. Growing cells were fixed with 3% formaldehyde and permiabilized 1% Nonidet P-40. As the 2nd antibody, goat anti-rabbit IgG conjugated with rhodamine was used. TAF1 protein was detected in nucleus.Enlarge Image

Immunofluorescence - Anti-TAF1 Antibody (A389)

Anti-TAF1 Antibody
Immunofluorescence staining of TAF1 protein in HeLa cells with anti-TAF1 antibody. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100. Anti-TAF1 antibody was used at 1/5,000 dilution. As a 2nd antibody, goat anti-rabbit IgG conjugated with Alex 488 was used at 1/1,000 dilution. Nucliei were stained with DAPI. The merged image was shown in the right panel. TAF1 protein was detected in nucleus.Enlarge Image

Immunofluorescence - Anti-TAF1 Antibody (A389)

Anti-TAF1 Antibody
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Proteins predicted to interact with TAF1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score