Anti-TAK1 (phospho Ser412) Antibody (A16432)

Western blot analysis of extracts of various cell lines, using Anti-TAK1 (phospho Ser412) Antibody (A16432) at 1:1,000 dilution. 293 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. K-562 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit.

Western blot analysis of extracts of various cell lines, using Anti-TAK1 (phospho Ser412) Antibody (A16432) at 1:1,000 dilution. C2C12 cells were treated by TGF-beta (10 ng/ml) at 37°C for 30 minutes. C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.