This antibody recognises the eta polypeptide of the CCT chaperonin molecule complex.CCT? is a 543 amino acid ~60 kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1)The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60 kDa in HeLa cell lysates using western blotting under reducing conditions.
Applications
WB, IHC-P
Dilutions
IHC-P: 1:100, WB: 1:500
Reactivity
Mouse, Human, Bovine, Rabbit
Host
Rat
Clonality
Monoclonal
Clone ID
PK/16/8/a
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Molecular Weight
Approximately 60 kDa in Hela cell lysates
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti CCTeta antibody, clone PK/16/8/a recognizes the eta polypeptide of the CCT chaperonin molecule complex.CCT? is a 543 amino acid ~60 kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1)The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60 kDa in HeLa cell lysates using western blotting under reducing conditions.
Synonyms
CCT-eta, CCT7, CCTH, Chaperonin containing T-complex polypeptide 1 subunit 7, HIV-1 Nef-interacting protein, NIP7-1, T-complex protein 1 subunit eta, TCP-1-eta
