Mouse monoclonal [MEM-262] antibody to TCR V beta 5.
Specificity
The antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Applications
Flow Cytometry, IP, WB, Functional Studies
Dilutions
WB: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting, FUNC: This antibody activates T cells (V beta 5-related subset), Flow Cytometry: 1-4 µg/ml.
Reactivity
Human
Immunogen
Human thymoma cell line HPB-ALL.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-262
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide (0.2 µm filter sterilized). Endotoxin level is less than 0.01 EU/µg of the protein.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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